Butterfield BrothPRINCIPAL USE: PRESERVE FIELD SAMPLES Used to dilute 1 mL of field sample (bulk water, biofilm, coupon) to prevent microbial cell rupture and change during the time between analysis and sampling. Sea water samples require a saline based broth to prevent osomotic shock. Sample should be chilled after collection and assayed within 8 – 24 hours depending on the growth phase of the microbes and/or the presence of biocides in the sample which may continue to work on the microbes while in transit. If biocide action and cell growth is in question, it is best to assay this sample with ATP for total microbes per mL (30 seconds) , or agar film plating method (48 hr.s), or for SRB's using API serum vials (14 –28 days). AMSA, Inc. provides all associated test kits. AMSA, Inc. provides DTEA, DBNPA, and select other biocides, DTEA biofilm removal products, and other specialty chemicals. PERFORMANCE: Representative samples of this lot were examined for cheinical/physical, sterility and neutralizing characteristics. Fill Volume: Fill volume clicks are performed by weight at regular intervals throughout production. **It should be noted that the graduations on the vials do not provide an accurate representation of the actual fill volume in tile Vials. Chernical/11hysical Parameters: Clarity/precipitate: Clear/None Final pH @ 25 deg. C (specification is 7.2 + 0.2): Avg. Fill Volume: 9.17 mL (8.5 - 9.5 nil) Terminal Sterilization Processing Parameters: This product has been subjected to Gamma irradiation as a terminal sterilization. process. The minimum and maximum dosages applied to this lot are as follows: Minimum Dose Level Delivered: 19.4 Maximum Dose Level Delivered: 29.0 At the minimum dosage, it is expected that 10.8 logs of microorganisms with Gamma inactivation profiles like Bacillus punilus (a spore-former with high resistance to Gamma irradiation with D = 1) = 1.8 kGy) will be inactivated. Toxicity/ Growth promotion: (quality check to ensure growth potential) The Toxicity/Growth Promoting capability of the dilutent is evaluated using low levels of organisms inoculated into the diluent and held @ 35 deg. C for one hour. In order to pass the Toxicity/Growth Promotion test, counts before and after incubation of the diluent must show no more than a 0.2 log difference. Escherichia coli ATCC 25922 Staphvlococcus aureus ATCC 29213 Log of initial = 2.06 Log at one hour = 2.15 colony counts = 2.00 colony counts = 2.11 APPROVAL STATUS: This lot was found to be satisfactory for all chernical/physical, sterility and neutralizing characteristics and was released for commercial sale. |
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